Fig. 6.

Histologic and functional Paneth cell measurements of germ-free Irgm1^−/− and wild-type mice. (A) Representative ileal tissue sections from germ-free (GF) wild-type (WT) and Irgm1^−/− (KO) mice (Lyz IHC, 20× magnification). (B) Number of Lyz⁺ cells per histologic crypt/villus unit. (C) Number of ectopic Lyz⁺ cells existing outside the base of the crypt per crypt/villus unit. (D) Average individual PC granule size. (E,F) Results of quantitative RT-PCR measurement of ileal tissue transcript levels of the antimicrobial peptides Lyz and Defa20 normalized to β-actin. n=6-12 mice/group, **P<0.005; error bars indicate s.e.m.; N.S., not significant.