Fig 2. Freund’s adjuvanted rHA protein vaccination elicits broader antibody mediated cross-reactivity compared to viral infection in ferrets.

The antibody response against homologuous and cross-reactive HA proteins from different influenza virus strains were evaluated by the mPlex-Flu assay. Samples included ferret reference antisera obtained from the Influenza Research Resource collected after viral infection (black) or after a prime-boost-boost vaccination protocol (see Methods) with Freund’s adjuvant admixed rHA (red). Dilutions of polyclonal antisera are provided in Table 1. The results calculated by subtracting the blank from mean MFI for each influenza virus strain with three replicate wells per influenza strain.