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. 2018 Apr 11;13(4):e0194891. doi: 10.1371/journal.pone.0194891

Fig 5. TRP120-1TR selectively binds to GC-rich DNA.

Fig 5

(A-B) HSQC spectra of 15N-labeled TRP120-1TR with GC-rich DNA at pH 7.0 or pH 5.5. TRP120-1TR (68 μM) alone (black) and with a 10-fold molar equivalent of unlabeled DNA (red, Table 1) in 20 mM Tris pH 7.0 containing 100 mM NaCl or in 10 mM phosphate pH 5.5 containing 100 mM NaCl are shown. The lines highlight Asn or Gln side chain NH2 peaks that show distinctly different chemical shifts in the bound form. (C-D) HSQC spectra of 15N-labeled TRP120-1TR with AT-rich DNA probe at pH 7.0 or pH 5.5. TRP120-1TR (30 μM) alone (black) and with a 10-fold molar equivalent of unlabeled DNA (blue, Table 1) in 20 mM Tris pH 7.0 containing 100 mM NaCl or in 10 mM phosphate pH 5.5 containing 100 mM NaCl are shown.