Electrically evoked (ES, 10 Hz) contractile activity of colonic longitudinal smooth muscle preparations isolated from mice fed with SD, SD plus treatment with apigenin (10 mg/Kg/day), HFD, or HFD treated with apigenin (10 mg/Kg/day). (A) Colonic tissues maintained in standard Krebs solution. (B) Colonic tissues maintained in Krebs solution containing guanethidine (10 μM) and L-NAME (100 μM). (C) Colonic tissues maintained in Krebs solution containing guanethidine (10 μM), L-NAME (100 μM), GR159897 (1 μM), SB218795 (1 μM) and atropine (1 μM). Values are means±SEM, n = 5. aP<0.05, bP<0.01 significant difference vs SD; *P<0.05, $P<0.01 significant difference vs HFD.