Fig 1. Construct with in vitro and in vivo expression of DEC-CX3CR1 and Con-CX3CR1 DNA vaccines.

(A) Open reading frames for mouse CX3CR1 extracellular domain were fused in frame to the C-terminus of scDEC or scControl vector. (B) The CX3CR1 PCR products were ligated and cloned separately into DEC205 (DEC-CX3CR1) and control vector (Con-CX3CR1), in agarose gels and visualized under UV using gel-doc 1000 (BIO-RAD, Australia, two separate bands as indicated in DEC.cut and Con.cut after restriction digestion). (C) Plasmid DNAs (scControl-CX3CR1, scDEC-CX3CR1, scControl vector, and scDEC vector) were transiently transfected into CHO cells and 24 hours post transfection total cell supernatant were subjected to western blot.