Table 1.
Primary antibody informationa
| Antibody | Host/isotype | Immunogen | Supplier/catalog #/lot #/RRID # | Validation | Dilution and application |
|---|---|---|---|---|---|
| Calbindin | Rabbit | Recombinant rat calbindin D-28k | Swant, Switzerland, catalog #CB38, RRID:AB_10000340 | Immunostaining in cerebellum of calbindin D-28k KO mouse (see datasheet) | IHC: 1:8000 |
| Glutamine synthetase | Mouse IgG2a | Human glutamine synthetase a.a. 1–373 | BD Biosciences, catalog #610517, RRID: AB_397879 | Recognizes a 45 kDa band on WBs of rat cerebrum lysates (see datasheet); expected pattern of staining in Müller glia | IHC: 1:10,000 |
| KV2.1 (K89/34) | Mouse IgG1 | a.a. 837–853 of rat KV2.1 | NeuroMab,b catalog #75-014, RRID: AB_10673392, lot #449-3AK-78 | WB in KV2.1 KO mouse (see datasheet) | IHC: 1:800 WB: 1:800 IP: 11.25 μg/ml protein |
| KV2.1 (L80/21) | Mouse IgG3 | a.a. 837–853 of rat KV2.1 | NeuroMab, catalog #73-315 RRID: AB_2315862, lot #437-6VA-52 | WB in KV2.1 KO mouse (see datasheet) | WB: 1:10 |
| KV2.2 | Rabbit | a.a. 859–873 of rat KV2.2 | Alomone Labs, catalog #APC120, RRID: AB_2040164, lot #AN-01 | WB with anti-KV2.2 antibody preincubated with the control peptide antigen (see datasheet); tested by immunohistochemistry in KO mouse (Tong et al., 2013) | IHC: 1:4000 IP: 11.25 μg/ml protein |
| KV2.2 (N372B/1) | Mouse IgG1 | a.a. 717–907 of rat KV2.2 long isoform | NeuroMab, catalog #73-369, RRID: AB_2315869, lot #437-8VA-10 | Immunostaining in KV2.2 KO mouse (see datasheet) | IHC: 1:8–1:10 WB: 1:8–1:10 |
| KV2.2 (N373B/60) | Mouse IgG2b | a.a. 717–907 of rat KV2.2 long isoform | NeuroMab, catalog #73-360, RRID: AB_2315867, lot #437-7VA-76 | WB and immunostaining in KV2.2 KO mouse (see datasheet) | IHC: 1:10 |
| KV2.2 (N372C/51) | Mouse IgG1 | a.a. 717–907 of rat KV2.2 long isoform | NeuroMab, catalog #73-358, RRID: AB_2315865, lot #437-7VA-73 | WB and immunostaining in KV2.2 KO mouse (see datasheet) | IHC: 1:10 |
| KV2.2 (K37/89) | Mouse IgG2a | a.a. 1–61 of human KV2.2 | NeuroMab, catalog #73-015, RRID: AB_10673393, lot #413-5RR-08; catalog #75-015, lot #443-1KS-100 | WB in KV2.2 KO mouse (see datasheet) | IHC: 1:50 |
| KV8.2 | Mouse IgG2b | a.a. 1–163 of mouse KV8.2 | NeuroMab, catalog #73-435, RRID: AB_2532091, lot #463-1DH-95 | Immunostaining in transfected cells expressing target and brain immunoblots (see datasheet) | IHC: mouse 1:2 |
| KV8.2 | Rabbit | a.a. 16–75 of human KV8.2 | Sigma-Aldrich, catalog #HPA031131, RRID: AB_10609996, lot #R31013 | Validated by protein array, single peak corresponding to KV8.2 antigen, immunostaining of human pancreas (see datasheet) | IHC: macaque and human 1:100 IP: 11.25 μg/ml protein |
| S-opsin | Goat | Peptide mapping at the N-terminus of the opsin protein encoded by OPN1SW of human origin | Santa Cruz Biotechnology, catalog #sc-14363, RRID: AB_2158332 | Pattern of staining consistent with expected ratio of S-cones; no cross-reactivity with cones expressing L/M opsin | IHC: 1:500 |
| L/M opsin | Rabbit | Recombinant human red/green opsin | Millipore, catalog #AB5405, RRID: AB_11212667, lot #2618475 | Pattern of staining consistent with expected ratio of L/M-cones; no cross-reactivity with cones expressing S opsin | IHC: 1:500 |
aa.a., Amino acids; IHC, immunohistochemistry; WB, Western blot; IP, immunoprecipitation.
bNeuroMab catalog numbers commencing 73- are tissue culture supernatants.