Fig 3. Northern blot analysis of pre-rRNA processing intermediates.

Utp24 shuffle strains transformed with a plasmid encoding wild-type or variant Utp24 were grown YPG and then shifted to YPD for 14 h. RNAs extracted from total cell lysate (TCL) and IgG immunoprecipitations (IP) were resolved in 1.2% agarose-formaldehyde gels and strained by ethidium bromide (EB). RNAs were transferred to Hybond N⁺ membranes, hybridized against ³²P-labeled probes and visualized by autoradiography. Asterisk and double asterisk denote non-specific bands of 25S and 18S rRNA, respectively.