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. Author manuscript; available in PMC: 2018 Apr 11.
Published in final edited form as: Exp Biol Med (Maywood). 2008 Mar 28;233(5):540–548. doi: 10.3181/0710-RM-265

Figure 3.

Figure 3

Effect of zinc supplementation on hepatocellular apoptosis in 129S6 mice chronically fed ethanol for 6 months. A. TUNEL assay of hepatocellular apoptosis was conducted using an ApoTag Apoptosis Detect kit. Scale bar: 100 μM. B. Quantitative measurements of TUNEL-positive cells in the liver. C. Activities of caspase-3 and -8 in the liver were colorimetrically measured by using Ac-DEVD-pNA as the caspase-3 substrate and Ac-IETD-pNA as the caspase-8 substrate. Results are means ± SD (n = 4–6). Significantly different (P < 0.05) among a, b, and c. Cont, control; EtOH, ethanol.