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. Author manuscript; available in PMC: 2018 Apr 11.
Published in final edited form as: Exp Biol Med (Maywood). 2008 Mar 28;233(5):540–548. doi: 10.3181/0710-RM-265

Figure 4.

Figure 4

Effect of zinc supplementation on oxidative stress in the liver of 129S6 mice chronically fed ethanol for 6 months. A. accumulation in the liver was detected by dihydroethidium that yields red fluorescence (arrows) in the nuclei upon oxidation by ROS. Scale 50 μM. B. Quantitative measurements of mitochondrial GSH and MT in the liver. GSH was measured using a Bioxytech GSH-400 assay kit, and MT was determined by a cadmium-hemoglobin affinity assay. Results are means ± SD (n = 4–6). Significantly different (P < 0.05) among a, and c. Cont, control; EtOH, ethanol. A color version of the figure is available in the online version of the article.