Fig. 2. Tools developed for the HEx platform.
(A) eGFP expression from a series of PADH2-like promoters in cultures grown under both fermentative (YPD) and respiratory (YPE) conditions. All fluorescence intensities are reported as the mean of three biological replicates. Error bars represent 1 SD (n = 3). (B) Four fungal BGCs, two controls and two previously uncharacterized systems, each produce improved titers when heterologously expressed using PADH2-like promoters as compared to strong constitutive promoters. ND, not detected. Error bars represent 1 SD (n = 3). Quantitation for TC1 and TC3 was based on the sum of the integrations of extracted ion counts corresponding to the oxidized sesquiterpenoids outlined in table S4.