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Schematic illustration of preparation and usage of GAG microarrays. GAG microarrays are most often prepared by spotting synthetic GAG oligosaccharides functionalized with amine at the reducing end onto NHS-activated glass slides. Solutions containing fluorescently labeled GAG-binding proteins are then incubated with the microarray. After the incubation, excess GAG-binding proteins are washed away and affinity of the protein for each spot is determined by the fluorescent intensity.
