Figure 4.

Changes in cell volume during incubation in the presence of sorbitol. Cells were incubated with 1.0 m sorbitol in the presence of 100 μm p-chloromercuriphenyl-sulfonic acid and in its absence. At designated times, a portion of each cell suspension was withdrawn and the cell volume was determined from measurements of EPR. The cell volume that corresponded to 100% was 0.75 ± 0.05 fL per cell in the absence of p-chloromercuriphenyl-sulfonic acid (●); in the presence of 100 μm p-chloromercuriphenyl-sulfonic acid (▴). Each point represents the average with se of results from four independent experiments.