Extended Data Figure 6. SRC-3 drives the purine synthesis program under conditions of active glycolysis.

a, MDA-MB231 cells stably expressing NTshRNA, shPFKFB4 and shSRC-3 were fed with [6-C¹³glucose]. Ribulose/Xylulose-5P (m+1) labeling from [6-C¹³glucose] were shown. [Mean ± s.d., n=3 biological cell samples, One-way ANOVA with Tukey’s Multiple comparisons test, ***P=0.00013; ****P=0.000078]. b, Genes involved in oxidative and non-oxidative PPP. [Mean ± s.d., n=3 biological cell samples, Two-way ANOVA with Sidak's multiple comparisons test, *P=0.0431]. c, Genes involved in nucleotide synthesis. [Mean ± s.d., n=3 biological cell samples, Two-way ANOVA with Sidak's multiple comparisons test]. d, Expression of metabolic enzymes transketolase (TKT), xanthine dehydrogenase (XDH), and adenosine monophosphate dehydrogenase 1 (AMPD1) in MDA-MB-231 cells transduced with adenovirus expressing GFP (control) and PFKFB4 cultured in presence of 5mM, 15mM or 25mM glucose. [Mean ± s.d., n=3 biological cell samples, two-way ANOVA with Dunnett's multiple comparisons test]. e–f, MDA-MB231 cells stably expressing NTshRNA, shPFKFB4 and shSRC-3 were fed with [6-C¹³glucose]. e, Seduheptulose-7P (m+1). and f, Erythrose-4P labeling from [6-C¹³glucose] were shown. [Mean ± s.d., n=3 biological cell samples, two-way ANOVA with Dunnett's multiple comparisons test (e) or with Tukey’s multiple comparison test (f), Boxes represent 25^th to 75^th percentile, line in the middle represents median, whiskers showing min to max all points]. For exact P-values please refer to source data.