Extended Data Figure 7. Growth defect due to loss of SRC-3 or PFKFB4 is rescued by exogenous purines.

a, Expression of metabolic enzymes transketolase (TKT), xanthine dehydrogenase (XDH), adenosine monophosphate dehydrogenase 1 (AMPD1) and SRC-3 in MDA-MB-231 cells expressing shRNA targeting control-shNT, shSRC-3-21 or re-expression of shRNA –resistant wildtype SRC-3 protein in SRC-3 depleted cells (shSRC-3-21+WT-SRC-3). [Mean ± s.d., n=4 biological cell samples, two-way ANOVA with Tukey’s Multiple comparisons test]. b, Relative proliferation of MDA-MB-231 expressing shRNA targeting SRC-3 (shSRC-3-1 and shSRC-3-2) or NT after treatment with siRNAs targeting luciferase (siLuc) as control or PFKFB4 under conditions indicated. [Mean ± s.d., n=6 samples from biologically independent experiments, two-way ANOVA with Tukey’s Multiple comparisons test, ****P<0.000001]. c, MDA-MB231 cells stably expressing NTshRNA, shPFKFB4 and shSRC-3 were fed with [U-C¹³glucose] for 48 hours. Adenosine C¹³ labeling from [U-C¹³glucose] were shown. [Mean ± s.d., n=3 samples from biologically independent experiments, one-way ANOVA with Tukey’s Multiple comparisons test; Boxes represent 25^th to 75^th percentile, line in the middle represents median, whiskers showing min to max all points]. Data shown are representative of 3 biologically independent experiments with similar results. For exact P-values please refer to source data.