Extended Data Figure 5. Increased glucose and purines are required for SRC-3 dependent growth.
a, Real time measurement of MCF10A cell proliferation transduced with adenovirus Adv. GFP or Adv. SRC-3 in presence of 93 different metabolites. n=3 independent plates run for each sample. Mean (center) ± s.d. (error bars), b, Relative growth of MCF10A cells transduced with Adv. GFP or Adv. SRC-3 in presence of D-glucose, c, adenosine and d, inosine. Mean ± s.d., n=6 biological cell samples, Unpaired t-test two tailed. [Boxes represent 25th to 75th percentile, line in the middle represents median, whiskers showing min to max all points]. e–f, Relative levels of intermediary metabolites in MDA-MB-231 cells after treatment with shRNAs targeting PFKFB4 or SRC-3 compared to control shRNA (NT). e, Glycolytic and pentose phosphate pathway (PPP) metabolites. f, Nucleotides. [Mean ± s.d., n=3 biological independent samples, two-way ANOVA with Tukey’s Multiple comparisons test, *P<0.05]. g, Total levels of purines in MCF10A cells transduced with Adv. GFP and Adv. SRC-3. [Mean ± s.d., n=3 biological independent samples, two-way ANOVA with Tukey’s Multiple comparisons test,; Boxes represent 25th to 75th percentile, line in the middle represents median, whiskers showing min to max all points]. For exact P-values please refer to source data.