Table 2. (A) Real-time PCR-based detection of gonorrhea in comparison to culture. (B) Detection of genetic markers associated with antimicrobial resistance in N. gonorrhoeae by real-time PCR.
Urethral swabs were first tested for the presence of gonorrheal DNA with the porA/opa multiplex assay. Gonorrhea-positive swabs were further analyzed for genetic markers associated with antimicrobial resistance by PCR. NT = not tested.
| A | Culture (+) | Culture (−) | ||
|---|---|---|---|---|
| PCR (+) | PCR (−) | PCR (+) | PCR (−) | |
| Number of swabs tested for gonorrhea (n = 210) | 68 | 33 | 5 | 104 |
| B | ||||
| Number of samples analyzed for resistance determinants | 62 | 0 | 5 | 47 |
| GyrA or ParC mutants | 15 | NT | 0 | 0 |
| GyrA mutants | 8 | |||
| ParC mutants only | 0 | |||
| GyrA and ParC mutants | 7 | |||
| PPNG | 1 | |||
| PBP-2 | 0 | |||