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. 2018 Jan 30;19(6):1427–1443. doi: 10.1111/mpp.12626

Figure 8.

Figure 8

Infection of Nicotiana benthamiana and Prunus persica plants by wild‐type Plum pox virus (PPV) and PPV mutants bearing phosphorylation‐mimicking mutations in the capsid protein (CP). Plants were inoculated by hand‐rubbing (N. benthamiana) or bombardment (P. persica) with viral cDNA. (A, C) Images of upper uninoculated leaves of N. benthamiana (A) or P. persica (C) plants after infection with the indicated viruses, taken at 21 or 23 days post‐inoculation (dpi), respectively, under an epifluorescence microscope. Bar, 5 mm. (B, D) PPV CP‐specific immunoblot analysis of extracts from upper uninoculated leaves of N. benthamiana (B) or P. persica (D) individual or pooled plants, collected at 21 dpi (N. benthamiana) or 23 dpi (P. persica). Inoculated viruses and extract volumes loaded are indicated. Blots stained with Ponceau red showing the ribulose‐1,5‐bisphosphate carboxylase/oxygenase (RuBisCO) protein are included as loading control.