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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Transl Stroke Res. 2017 Oct 11;9(3):215–222. doi: 10.1007/s12975-017-0572-0

Fig. 7.

Fig. 7

A: Ubiquitin immunogold EM micrographs of mitochondrion (M). EM micrographs of CA1 neurons from a sham-operated non-ischemic control rat and a rat subjected to 15 min ischemia followed by 24h of reperfusion. Ubiquitin immunogold labels mitochondrion and ubiquitinated protein aggregates at 24h of reperfusion (arrows). B: EM three-dimensional reconstruction of CA1 neuronal mitochondria in brain sections from sham-operated control and 24h reperfused rats. The red indicates the mitochondrial outer membrane. The yellow (arrows) represents the vacuole structures formed between the inner- and outer-mitochondrial membranes after brain ischemia. Scale bars in A and B = 0.5 μm. C: Western blotting of ubiquitin in the samples of detergent-salt insoluble mitochondrial pellets from sham-operated rats and rats subjected to 15 min of ischemia followed by 24 h of reperfusion. The molecular size is shown on the left.