Fig.1. LRRK2 preferentially interacted with Rac1and Rab29.

A: Co-Immunoprecipitation of EGFP-Rab29 or EGFP-Rac1 with 3HALRRK2^WT overexpressed in COS7 cells. The data indicated that Rac1 and Rab29 specifically interacted with LRRK2. B: Schematic representation of LRRK2 constructs used. C&D: Co-Immunoprecipitation of GFP-Rac1 (C) or 3Flag-Rab29 (D) with different fragments of 3HA-LRRK2 (full length, Roc, COR or Kinase domains) overexpressed in COS7 cells. The data indicated that Rac1 specifically bound to COR and kinase domain while Rab29 mainly interacted with COR domain of LRRK2. E&F: Quantification analysis of the relative Rab29 levels (normalized by the WT) was presented in C and D, Data presented were mean±SEM from two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA followed by Tukey's post hoc test.