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. 2018 Mar 26;32(2):145–156. doi: 10.7555/JBR.31.20170039

Fig.2. Rab29 but not Rac1 acts as downstream of LRRK2 signaling in regulating retrograde trafficking of CI-M6PR.

Fig.2

A: Double immunostaining of HeLa Swiss cells transiently expressing Myc-CI-M6PR with wild type, G2019S or K1906M of 3HA-LRRK2, respectively. The data showed that LRRK2G2019S altered the perinuclear localization of CI-M6PR with diffused staining pattern. B: Double immunostaining of HeLa Swiss cells transiently expressing Myc-CI-M6PR and wild type or mutant 3Flag-Rab29, respectively. The data showed that Rab29 mutations (CA and DN) altered the perinuclear localization of CI-M6PR. C: Double immunostaining of HeLa Swiss cells overexpressing Myc-CI-M6PR and wild type or mutant EGFP-Rac1, respectively. The data showed that Rac1 did not alter the perinuclear localization of CI-M6PR. Fluorescence intensity profiles of stained proteins were shown in the green and red channels of the regions indicated by the white lines. The CIM6PR fluorescence intensity near the nucleus represents the quantitative perinuclear distribution. Scale bar = 10 μm.