Skip to main content
. 2018 Mar 26;32(2):145–156. doi: 10.7555/JBR.31.20170039

Fig.3. Overexpressed Rab29 mutants but not Rac1 accelerated CI-M6PR degradation.

Fig.3

A-C: CI-M6PR was co-transfected in HeLa Swiss cells with indicated plasmids and the cells were treated with 100 mg/mL CHX and collected samples at the indicate time points (0, 6 and 12 hours) for detecting the levels of the overexpressed proteins. The data suggested that LRRK2G2019S reduced the half-life of CI-M6PR compared with that in wild type or LRRK2K1906M group (A). Furthermore, overexpressed mutant Rab29s but not Rac1 reduced the half-life of CI-M6PR compared with that in wild type group (B&C). The arbitrary densitometry value in A, B and C was measured using imaging analysis software Image J. Data (mean±SEM) were from the indicated number of independent experiments and comparisons were analyzed usingone-way ANOVA followed by Tukey's post hoc test. *P < 0.05, **P < 0.01 and ***P < 0.001.