Fig.4. Rab29 but not Rac1 is a downstream effector of LRRK2 involved in retrograde trafficking of CI-M6PR.

A: HeLa Swiss cells overexpressing Myc-CI-M6PR, 3HA-LRRK2^G2019S and 3Flag-Rab29^WT or EGFP-Rac1^WT were immunostained, which indicated that Rab29 but not Rac1 could rescue the altered subcellular distribution of CI-M6PR induced by 3HA-LRRK2^G2019S. B: Co-overexpression of LRRK2^G2019S and Rab29^WT or Rac1^WT HeLa in Swiss cells was used to determine the half-life of CI-M6PR. The data showed that Rab29^WT could rescue the reduced half-life of CI-M6PR induced by LRRK2G2019S. C: Quantitative analysis of the relative CI-M6PR levels (normalized by β-actin, time point 0 hour) in (B). Data (mean±SEM) were from the indicated number of independent experiments and comparisons were made using one-way ANOVA followed by Tukey's post hoc test. *P < 0.05, **P < 0.01 and ***P < 0.001.