Fig.5. Both Rab29 and Rac1 rescued neurite shortening induced by LRRK2^G2019S in differentiated SH-SY5Y cells.

A: Overexpressed 3HA-LRRK2^G2019S in differentiated SH-SY5Y cells reduced neurite length compared with that for wild type protein. Na⁺-K⁺ ATPase was used to track plasma membrane of the cells. B: Co-overexpression LRRK2^G2019S with Rab29^WT or Rab29^CA but not Rab29^DN could significantly rescue neurite retraction induced by LRRK2G2019S. C: Co-overexpression LRRK2G2019S with Rac1^WT or Rac1^CA but not Rac1^DN could significantly rescue neurite retraction induced by LRRK2^G2019S. These results suggested that Rab29 and Rac1 can act as downstream of LRRK2 mediated neurite outgrowth. Quantification analysis of the relative neurite length (normalized by the WT) was presented in A, B, and C. Data presented were mean±SEM from two independent experiments. *P < 0.01 and ***P < 0.001, unpaired t-test.