Fig. 2.

L5 likely templates folding of the Pet passenger domain by β-strand augmentation. Topology model of the Pet β-barrel domain showing the Pet^L5β1/G, Pet^L5β2/G, and Pet^L5Un/G (a), and the Pet^L5FadL, Pet^L5OmpF, and Pet^L5β1/P (b) mutations (in red). Pulse-chase expression of Pet, Pet^L5β1/G, Pet^L5β2/G, and Pet^L5Un/G (c), and of Pet, Pet^L5FadL, Pet^L5OmpF, and Pet^L5β1/P (d), and sensitivity to proteinase K (PK) in E. coli TOP10 monitored by SDS-PAGE and immunoblotting with anti-Pet passenger domain antibodies. All samples were TCA precipitated prior to SDS-PAGE. Images are representative of at least two independent experiments