Table 1.
Summary and yield of ND- and GH-porcine intestinal mucin preparations after two-step isopycnic density gradient centrifugation.
| Preparation | Total protein (μg) | Mucin (μg) | Protein (μg) | Protein | Net Muc2 yield (%) | |
|---|---|---|---|---|---|---|
| Gross (%) | Net (%) | |||||
| Native | 10 | 9.27 | 1.61 | 16.1 | 11.29 | 92.68 |
| Denatured | 10 | 9.88 | 0.86 | 8.56 | 3.75 | 98.83 |
| Denatured (diafiltered) | 10 | 10 | 0.48 | 4.8 | 0 | 100 |
A PAS-microtitre assay was used to determine quantitatively the glycoprotein concentration using diafiltered GH-mucin preparation as a standard. Results shown represent the mean of analytical triplicates.