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. 2018 Jan 16;21(1):137–146. doi: 10.1038/s41391-017-0016-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Western blot analysis for PSA and FUT8 in LAPC4 cells that were transfected with plasmid carrying wild-type FUT8 or shRNA against FUT8 or vector control. PC3 cells were included as a positive control for FUT8 and negative control for PSA expression. Beta actin was included to ensure equal amount of loading across the lanes (a). Inverse association between FUT8 and PSA expression in 10 metastatic prostate tumor samples (R = −0.321). AAL blot was included to confirm the functional increase of FUT8 in all 10 samples (b) (online color figure)