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. 2018 Jan 25;37(15):1991–2007. doi: 10.1038/s41388-017-0081-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. If you remix, transform, or build upon this article or a part thereof, you must distribute your contributions under the same license as the original. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/.

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Fig. 2 — Inducible knockdown of PAX3-FOXO1 decreases proliferation, migration, and invasion of FP-RMS. a Schematic of PAX3-FOXO1 translocation and location of shRNA expressed by doxycycline-inducible Tet-pLKO lentivirus. b Expression of PAX3-FOXO1 by immunoblot with FOXO1 directed antibody in Rh41 cells stably transduced with Tet-pLKO-P3F or Tet-pLKO-Scr treated with and without doxycycline for 5 days. c Expression of PAX3-FOXO1 and transcriptional targets of PAX3-FOXO1 by qRT-PCR in Rh41 cells as in (b). d PAX3-FOXO1 knockdown decreases population doubling of Rh41 cells. e Cell Titer Glo and f Caspase 3/7 Glo assays in cells from (b) treated with doxycycline for 3 days. g Cell cycle analysis of cells from (b) after 5 days of doxycycline treatment. h Low-density colony formation: i cell migration and j invasion in Rh41 PAX3-FOXO1 knockdown cells. *P < 0.05, **P < 0.01, ***P < 0.001 for shScr+Dox vs shP3F+Dox