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. 2018 Jan 25;37(15):1991–2007. doi: 10.1038/s41388-017-0081-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. If you remix, transform, or build upon this article or a part thereof, you must distribute your contributions under the same license as the original. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/.

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Fig. 3 — Identification of microRNAs regulated by PAX3-FOXO1. a Heat map comparing differential expression of microRNAs in stably transduced Rh41 cells with Tet-pLKO-P3F or Tet-pLKO-Scr and treated with doxycycline for 5 days, and RD cells stably transduced with PAX3-FOXO1 or empty control vector. Downregulated genes indicated in blue (−1.5 SD) and upregulated genes indicated in red (1.5 SD) with FDR < 0.05. b Validation of microarray by qRT-PCR for microRNAs downregulated with PAX3-FOXO1 knockdown in Rh30 and Rh41 Tet-pLKO cells treated with doxycycline for 5 days. c Expression of PAX3-FOXO1 regulated microRNAs by qRT-PCR in RD and LHCN myoblasts stably transduced with control empty vector or PAX3-FOXO1 overexpression. d Expression of miR-221 by qRT-PCR in PAX3-FOXO1 knockdown cells treated with doxycycline for 5 days or e stably transduced RD or LHCN myoblasts. *P < 0.05, **P < 0.01, ***P < 0.001 for shScr+Dox vs shP3F+Dox or empty vs PAX3-FOXO1