Fig. 6.

PAX3-FOXO1 regulates miR-486-5p expression. a Map of the genomic location of miR-486-5p within the ANK1 gene downstream of the alternative exon 39A of the sANK1 isoform. b Expression of sANK1 by qRT-PCR in RD or LHCN cells stably transduced with empty control vector or PAX3-FOXO1. c Expression of sANK1 by qRT-PCR in Rh30 or Rh41 shRNA knockdown cells after doxycycline treatment for 5 days. d Luciferase activity in 293T cells co-transfected with the pGL3-sANK1-Promoter reporter vector and either Empty control, MyoD, or increasing dose of PAX3-FOXO1 expression vectors. *P < 0.05, **P < 0.01, ***P < 0.001 shSCR vs shP3F, or Empty vs P3F