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. 2018 Apr 11;9:1394. doi: 10.1038/s41467-018-03895-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Transfection of FLAG tagged histone H2A mutants and their influence on H2BK120ub in HEK 293T cells. a Experimental procedure and representative western blot (WB) analysis. b Quantification of WB analysis (αH2BK120ub/αH3) plotted relative to wt. Data are mean ± SEM (n = 4 for K120R; n = 5 for all other samples). (Full western blot images are presented in Supplementary Fig. 10b)