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. 2018 Apr 11;8:5860. doi: 10.1038/s41598-018-23985-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mulberry FLOWERING LOCUS C-like genes. (A) Phylogenetic relationship of FLC homologs from multi-species. Phylogenetic tree of FLC homologs is generated using PhyML (version 3.1) by maximum likelihood method with GTR + I + G model. The mulberry genes are marked in red. (B) Analyses of subcellular localization of MnMADS33, MnMADS50, and MnMADS7 in Nicotiana benthamiana leaves. Agrobacterium-infiltrated tobacco leaves, expressing the MnMADS33-GFP, MnMADS50-GFP, and MnMADS7-GFP fusion proteins driven by the CaMV 35S promoter, were used for obtaining images under green fluorescence, merged light, and visible light. 35S:00 represent the empty expression vector. Scale bar = 25 μm. (C) Comparison of A. thaliana FLC, MnMADS33, MnMADS50, and MnMADS7 amino acid sequences. The MADS-box, I, K, and C-terminal domains are labelled.