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. 2018 Apr 11;8:5860. doi: 10.1038/s41598-018-23985-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of MnMADS33 and MnFT genes in floral buds. (A,B) Expression of MnMADS33 and MnFT in adult mulberry buds over one year. (C,D) Expression analyses of MnEXP2 and MnCYCD3 in the mulberry floral buds during endodormancy. (E,F) Expression of MnMADS33 and MnFT in mulberry floral buds under controlled cold conditions in 30 days. Gene expression is measured by qRT-PCR using the RPL15 as a reference (n = 3, mean ± measurement range).