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. 2018 Apr 11;8:5812. doi: 10.1038/s41598-018-24228-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression analysis in monocytes and macrophages. (A,B) Relative qPCR analysis of CD68 and iNOS. (C) RT-PCR analysis of Arg-1 and ß-actin in monocytes and macrophages of B6 and C3H mice. Images of Arg-1 and ß-actin were taken from 2 different parts of the same gel. The Numbers 1 to 3 stand for sample number. Expression of CD68 was used as a marker for the macrophage lineage, while iNOS expression was used to indicate M1 polarization, and Arg-1 expression was used to indicate M2 polarization (A,C). Monocyte-derived macrophages from B6 and C3H mice expressed equal levels of CD68, indicating equivalent differentiation to the macrophage lineage (A). C3H macrophages expressed higher levels of the M1 marker iNOS (B) and the M2 marker Arg-1 than B6 macrophages. No expression of Arg-1 was detected in B6 macrophages (C).