Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 11;8:5812. doi: 10.1038/s41598-018-24228-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Expression of complement components and receptors and ICAM-1. (A) C3a, C3ar, and C5ar expression in monocytes and macrophages. (B) Gene expression analysis in MN cells. (C) Images of C5a and ß-actin were taken from 2 different parts of the same gel. C5a expression was not detected by RT-PCR in monocytes, macrophages, or MN cells of B6 or C3H mice. Liver tissue was used as a positive control. In DCC-susceptible C3H mice, C3a and C3ar were highly expressed in the early stages of cell differentiation, but expression of C3 increased much more slowly during macrophage differentiation. C3a and C3ar expression in MN cells was not significantly different between the strains. No expression of C5a was detected in either group (C), although the MN cells of B6 mice tended to express higher levels of C5ar (B,D) ICAM-1 release from macrophages of B6 and C3H mice was analyzed by ELISA. Higher release of ICAM-1 was observed from the macrophages of calcification-susceptible C3H mice. (E) Expression of ICAM-1 in calcifying myocardial tissue from C3H mice compared with non-calcifying tissue from B6 mice following freeze–thaw injury.