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. 2018 Apr 11;8:5812. doi: 10.1038/s41598-018-24228-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Serum factors from DCC-resistant B6 mice inhibit MN cell formation in DCC-susceptible C3H mice. Splenic monocytes from C3H (A) or B6 (B) mice were differentiated into MN cells, and the serum in the culture supernatant was replaced with plasma from either C3H or B6 mice. MN formation was analyzed microscopically by TRAP staining. TRAP-positive cells with more than three nuclei were counted and quantified.