FIGURE 1.
MAB_4384 is a specific repressor of the mmpS5/mmpL5 locus in M. abscessus. Transcriptional profile of 19 mmpL in M. abscessus expressed in fold induction relative to expression in the wild-type strain (CIP104536T) in (A) the D15_S4 spontaneous mutant resistant to TAC analogs containing a stop codon in MAB_4384 and in (B) MAB_4384::pUX1 in which MAB_4384 has been disrupted by homologous recombination. tgs1 was included as a non-relevant control gene. Error bars indicate standard deviation. (C) Expression of MAB_4384 in M. abscessus. Fold induction levels of MAB_4384 were calculated in MAB_4384::pUX1 relative to the parental strain. Error bars indicate standard deviation. Relative gene expression was calculated using the ΔΔCt method with correction for PCR efficiency. Data is representative of three independent experiments.