Fig. 4.

Genetic correlations between the dwarf phenotype in dw and GmDW1 (Glyma.08G163900). a Genomic sequence of the GmDW1 allele among dw, Zp661, and the Williams 82 reference genome was compared; a T-to-A change in the third exon was detected in dw, and two additional allelic variations (Gmdw1-1 and Gmdw1-2) in GmDW1 were also screened from soybean accessions. b The SNP locus (T>A) in Glyma.08G163900 was linked to the mutant phenotype in dw, when genotyping each individual in the resequenced mutant or wild-type pool. c Relative expression level of GmDW1 was detected by qPCR, with data normalized to Actin11 levels (n = 3) (Cook et al. 2012), in different tissues including stem, leaf, and root from 2-week-old dw and Zp661 plants