Fig. 5.

The relative expression of GA metabolic pathway-related genes in dw and the parental line Zp661. The expression level of GA biosynthesis-related genes GA-20 oxidase (a Glyma.09G149200, b Glyma.20G153400) and CPS (Copalyl pyrophosphate synthase) (c Glyma.19G157000) homologues in soybean was examined in stems of 14-day-old dw and Zp661 seedlings. d–f The relative expression of GA response-related genes GID1a (d Glyma.20G230600) and RGA (e Glyma.05G140400, f Glyma.11G216500) homologues in soybean in stems of two-week-old dw and Zp661 plants. The soybean Actin11 gene (Glyma.18G290800) was used as the internal control (Cook et al. 2012), and three replicates were performed for each of the genes in a–f. The relative transcript abundance for these genes in a-f against the soybean Actin11 gene (Glyma.18G290800) was quantified using the $2^{-\mathrm{\Delta }\mathrm{\Delta }{C}_{\text{T}}}$ method (Livak and Schmittgen 2001). All the primers for qPCR in a–f are shown in Table 2