Table 2.
GA mechanism-related genes in soybean and the primers for qPCR analysis
| Some identified GA mechanism-related genes in plants | Soybean homologs | Primer ID | Forward strand sequence (5′–3′) | Reverse strand sequence (5′–3′) |
|---|---|---|---|---|
| GAs biosynthesis-related genes from Arabidopsis and Medicago truncatula | ||||
| Copalyl pyrophosphate synthase (CPS) (AT4G02780) | Glyma.19G157000 | CPS-2 | ACTGCCACCTTCCCTCTTTC | TGTTTGTCGTTAGTCTCGGAC |
| GA-20 oxidase (AT4G25420) | Glyma.09G149200 | GA-1 | GATAGAGAGACCCTGTGCCT | TGAGAAGCAGAGCAAAACAGAG |
| Glyma.20G153400 | GA-2 | TGGCTGCAACGGAAAAGTAA | TAGCCCCATAGCCCTACTCA | |
| Ent-kaurene synthase (KS) (MTR_2G064295) | Glyma.08G163900 | RT08-5 | ATGTGCTGGCTTTGCGTATT | CCTTGCACTCTCTGGGAACT |
| GA-responsive genes isolated in Arabidopsis or Medicago truncatula | ||||
| GID1a (MTR_8G035520) | Glyma.20G230600 | GR-2 | AGTTCCTGTATCCCTGTGCC | TGGCAGGGAAAGAGAAGAGG |
| RGA (AT2G01570) | Glyma.05G140400 | GR-6 | CTGGCTCCAAACCATGCTTT | CCCCGGAATAGCCTTGAGAT |
| Glyma.11G216500 | GR-8 | TCCCCAGATCGTTACCATCG | TCCCAAGGTACAACTCGGAC | |
| Reference gene | ||||
| The soybean Actin11 gene | Glyma.18G290800 | Actin11 | ATCTTGACTGAGCGTGGTTATTCC | GCTGGTCCTGGCTGTCTCC |