Table 8.
Phenotypic analysis and allelic variations in GmDW1 from soybean accessions
| Materialsa | Sequence variation and genotype | SNP effect | Plant height at maturity (cm)c | P valued | Average internode length (cm)c | P valued |
|---|---|---|---|---|---|---|
| Cultivated soybean accessions | ||||||
| ZDD23269 | G-to-A change at nucleotide 5811/Gmdw1-1 | Arg to His at residue 623 | 102.1 ± 4.3 (n = 10) | – | 5.2 ± 0.2 | – |
| ZDD23893 (Zp661) | Wildtype/GmDW1 | Wildtype | 130.9 ± 4.7 (n = 10) | < 0.0001 | 5.8 ± 0.4 | 0.0003 |
| ZDD02315 | Wildtype/GmDW1 | Wildtype | 160.0 ± 11.8 (n = 5) | 0.0111 | 8.8 ± 1.2 | 0.0330 |
| ZDD12910 | Wildtype/GmDW1 | Wildtype | 171 ± 24.1 (n = 5) | 0.0101 | 6.6 ± 0.9 | 0.0455 |
| ZDD03651 | Wildtype/GmDW1 | Wildtype | 180.6 ± 10.4 (n = 5) | < 0.0001 | 8.4 ± 0.2 | < 0.0001 |
| Wild soybean accessions | ||||||
| ZYD02878 | G-to-A change at nucleotide 2837 and 5811/Gmdwb (Gmdw1-1 and Gmdw1-2) | Val to Ile at residue 439 and Arg to His at residue 623 | 7.9 ± 1.7 (n = 6) | – | 1.4 ± 0.3 | – |
| ZYD03687 | Wildtype/GmDW1 | Wildtype | 46.0 ± 15.2 (n = 10) | < 0.0001 | 5.2 ± 1.3 | < 0.0001 |
| ZYD04569 | Wildtype/GmDW1 | Wildtype | 34.5 ± 9.7 (n = 15) | 0.0006 | 3.1 ± 0.8 | 0.0011 |
| ZYD04638 | Wildtype/GmDW1 | Wildtype | 54.2 ± 13.6 (n = 6) | 0.0004 | 4.4 ± 1.2 | 0.0012 |
aCultivated and wild soybean accessions are given a “ZDDxxxxx” or “ZYDxxxxx” number, respectively, and conserved in the National Crop Gene Bank, Chinese Academy of Agricultural Sciences
bThe Gmdw genotype contained both the Gmdw1-1 and the Gmdw1-2 allele
cFor each accession, 5–10 plants (n) were measured for plant height and internode length. All data are given as mean ± SD
dP values for differences between the soybean accession with GmDW1 genotype and the soybean accession with Gmdw1-1 or/and Gmdw1-2 allele were generated by a Student’s t test