FIGURE 2.

Predicted minimum free energy conformations of the regions around the ligation site of wild-type ELVd and different mutants. C197A, G198U, C200G, and G201A are punctual nucleotide substitution mutants, and ΔU194-G204, ΔC197-G201, ΔG1-U7, ΔG38-U42, ΔU47-G56, and ΔG317-U326 are deletions mutants. Nucleotide positions refer to ELVd sequence variant AJ536613. Mutated nucleotides and nucleotides on the border of deletions are highlighted in red. The terminal nucleotides ligated during circularization are in blue. The 5′-hydroxyl and 2′,3′-phosphodiester groups of the terminal nucleotides are indicated by –OH and >P, respectively.