Table 1.

Primers used to mutagenize the ELVd cDNA.

Mutant	Sequencebbb
C197A	5′-GGCGGAAGACGCTTTCAGACGGTGGGTTCGTCGAC-3′ 5′-CCGCGAAGACCGGAAAGTGTGTACTTTCCCTG-3′
G198U	5′-GGCGGAAGACGCTTTCCTACGGTGGGTTCGTCGAC-3′ 5′-CCGCGAAGACCGGAAAGTGTGTACTTTCCCTG-3′
C200G	5′-GGCGGAAGACGCTTTCCGAGGGTGGGTTCGTCGAC-3′ 5′-CCGCGAAGACCGGAAAGTGTGTACTTTCCCTG-3′
G201A	5′-GGCGGAAGACGCTTTCCGACAGTGGGTTCGTCGAC-3′ 5′-CCGCGAAGACCGGAAAGTGTGTACTTTCCCTG-3′
ΔU194-G204	5′-GGCGGAAGACGCCACTGGTTCGTCGACACCTCTCCC-3′ 5′-CCGCGAAGACCGAGTGTGTACTTTCCCTGATG-3′
ΔC197-G201	5′-GGCGGAAGACGCTTTCGTGGGTTCGTCGACACCTC-3′ 5′-CCGCGAAGACCGGAAAGTGTGTACTTTCCCTG-3′
ΔG1-U7	5′-TATGGGGCAGCGTTACAAGT-3′ 5′-GTGTGCCACCCCTGATGAGAC-3′
ΔG38-U42	5′-GACCTTTCGGTCTCATCAGG-3′ 5′-GGGGTTTCGCCATGGGTCGG-3′
ΔU47-G56	5′-CCCCATTTCGACCTTTCGGTC-3′ 5′-GGTCGGGACTTTAAATTCGG-3′
ΔG317-U326	5′-CTCTATCTCTCCTGGAAGGC-3′ 5′-CCCCATAGGGTGGTGTGTGC-3′

bbb BpiI recognition and cleavage sites are on a gray background and underlined, respectively. The punctual nucleotide substitutions are in italics and bold.