Figure 1.

Cellular content of pleural fluid after lung resection (solid line) and lung transplantation (dotted line). When clinically available, samples were obtained from N = 45 patients; N = 37 patients after lung resection; and N = 8 patients after lung transplantation. (A) After red cell lysis, cell concentrations were determined by manual counting with a hematocytometer. (B) Cell viability was determined by light microscopy and trypan blue exclusion (27) as well as stage scan fluorescent microscopy after calcein-AM and ethidium homodimer-1 cell viability staining. Error bars reflect ± 1 SD.