Figure 2.

Flow cytometry profile of cells in the pleural fluid. After exclusion of debris and red cells, the remaining cells were studied by flow cytometry. (A) Anti-CD45 staining and side-scatter (SSC) analysis demonstrated a dominant mononuclear population. A representative sample on day 1 after surgery demonstrated 70% mononuclear cells and 5% granulocytes; the remaining 25% of cells were compatible with mesothelial cells. Unstained negative controls were compared to samples stained with detection antibodies (B,C). More detailed size analysis with forward light scatter (FSC) on postoperative day 1, 3, and 7 showed no consistent size relationship between CD45⁺ cells (blue) and CD45⁻ cells (red; the Emphasize Plot Tool, FCS Express, was used for data presentation). Representative histograms are shown.