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Fluorescence immunohistochemistry of human pleural mesothelium. Human pleura, obtained from surgical specimens, was stained with fluorochrome-labeled anti-CD71 (A), anti-WT1 (B), and anti-CD45 (C) monoclonal antibodies and counterstained with Hoechst 33342. Both anti-CD71 and anti-WT1 antibodies demonstrated discontinuous staining of the pleural mesothelium. There was no detectable pleural staining with labeled anti-CD45 monoclonal antibody. Bar = 50 um.
