Table 1.

The constituents of M15 medium, a defined medium for the growth of L. crescens.

Components	M15
Inorganic salts
Calcium chloride dihydrate	1320
Magnesium chloride anhydrous	1068.2
Magnesium sulfate anhydrous	1356.7
Potassium chloride	2240
Sodium phosphate monobasic monohydrate	1007
Amino acids
β-Alanine	447.25
L-Alanine	447.25
L-Arginine-HCl	1777
L-Asparagine monohydrate	1075.45
L-Aspartic acid	818.6
L-Cystine-2HCl	56.38
L-Glutamic acid	1502.2
L-Glutamine	358.04
Glycine	859.512
L-Histidine hydrochloride monohydrate	2366.11
L-Isoleucine	687.36
L-Leucine	592.89
L-Lysine-HCl	1464.85
L-Methionine	678.9
L-Phenylalanine	789.62
L-Proline	940.61
DL-Serine	944.76
L-Threonine	459.8
L-Tryptophan	373.73
L-Tyrosine disodium salt	391.37
L-Valine	644.31
Betaine	0.31
Ornithine	229.96
Methionine sulfoxide	18.2
Vitamins + Others
L-Biotin	0.1
Choline chloride	1000
Folic acid	0.2
Myo-inositol	0.2
Niacin	0.2
D-Calcium pantothenate	0.2
Para-aminobenzoic acid (PABA)	0.2
Pyrodoxine-HCl	0.2
Riboflavin	0.2
Thiamine-HCl	0.2
Organic acids
Citric acid	2500
pH	5.92

Concentrations are given in mg/L.