Table 2.
Results of confirmatory tests of positive samples by the BP-MS method.
| Target bacteria | No. (%) of positive samples by BP-MS | No. of samples confirmed by real-time PCR/No. of positive samples by BP-MS | Verification rate (%) of real-time PCR | No. of discordant samples confirmed by nested PCR | No. of overall confirmed samples | Overall verification rate (%) |
|---|---|---|---|---|---|---|
| Streptococcus pneumoniae | 124 (60.8) | 72/124 | 58.1 | 40 | 112 | 90.3 |
| Haemophilus influenzae | 56 (27.5) | 52/56 | 92.9 | 2 | 54 | 96.4 |
| Neisseria meningitidis | 1 (0.5) | 0/1 | 0 | 1 | 1 | 100 |
| Klebsiella pneumoniae | 25 (12.3) | 1/25 | 4 | 15 | 16 | 64 |
| Acinetobacter baumannii | 23 (11.3) | 11/23 | 47.8 | 7 | 18 | 78.3 |
| Pseudomonas aeruginosa | 13 (6.4) | 9/13 | 69.2 | 2 | 11 | 84.6 |
| Staphylococcus aureus | 25 (12.3) | 17/25 | 68 | 7 | 24 | 96 |
| Moraxella catarrhalis | 27 (13.2) | 24/27 | 88.9 | 2 | 26 | 96.3 |
| Legionella pneumophila | 2 (1) | 1/2 | 50 | 0 | 1 | 50 |
| Mycoplasma pneumoniae | 25 (12.3) | 21/25 | 84 | 1 | 22 | 88 |
| Bordetella pertussis | 3 (1.5) | 3/3 | 100 | 0 | 3 | 100 |
| Totala | 167(81.9) | 77/167 | 46.1 | 59 | 136 | 81.4 |
a
The total number of clinical samples is 204.