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. 2018 Apr 11;8:5811. doi: 10.1038/s41598-018-23832-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Repertoire of histone chaperones and mobility of histones are limited in dTSCs. (a) Expression of genes encoding histone chaperones. Graphs show the expression level of various histone chaperones determined using RT-qPCR. Values indicate the mean ± S.D. after being normalized to Actb expression, and are indicated relative to the levels in TSCs, which were arbitrarily set as 1. (b,c) FRAP analysis for H4-GFP-TSCs and -dTSCs at day 8. One-half of the nuclei of living H4-GFP-TSCs and -dTSCs at day 8 was bleached (white squares in b), and the averages of the relative fluorescence intensity (mean ± S.D.) of the bleached area up to 55-min post-bleaching are plotted (n = 10) (c). Bars = 5 μm.