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. 2018 Apr 11;8:5858. doi: 10.1038/s41598-018-24256-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Wound healing assay of siRNA knockdown in MDA-MB-231 and PANC1 cells. (A) Western Blot demonstrating knockdown of PHB1, PHB2, and ILK1 in MDA-MB-231 and PANC1 cells. (B and C) siRNA knockdown of PHBs or ILK1 inhibits migration of different types of cancer cells. Each gap was created in confluent cells transfected with siRNA against PHBs or ILK1 and the evolution of the gap width was measured over time. Scale bar = 200 μm.