Table 2.
Distances between segregated nucleoids in deeply-constricted cells from slow and fast growing populations of E. coli MC1000.
| Growth medium (Td min) | >Cells with two nucleoids |
>Distances (μm) between nucleoids in deeply-constricted cells (diameter at constriction site < mean diameter at constriction site; see Table 1, columns 11 to 14) |
||||
|---|---|---|---|---|---|---|
| % (cell count) | S-period(1), min | >Measured manually(2) |
>Distance between centers of mass measured from profile plots(3) |
|||
| Small cells mean ±SD (cell count) | Large cells mean ±SD (cell count) | Small cells mean ±SD (cell count) | Large cells mean ±SD (cell count) | |||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| Succinate (122 min) | 17 (1017) | 24 | 0.56 ± 0.11 (24) | 0.70 ± 0.14 (26) | 1.26 ± 0.33 (25) | 1.72 ± 0.21 (26) |
| Glucose+aa (29 min) | 74 (747) | 23 | 0.74 ± 0.12 (63) | 0.89 ± 0.14 (56) | 1.79 ± 0.15 (63) | 2.21 ± 0.22 (56) |
(1)The period, S, during which cells contain two separated nucleoids, was calculated from S = Td.ln(1+F)/ln2, in which F is the fraction of cells with two nucleoids (based on Bremer and Churchward, 1977). (2)Measured manually with ObjectJ program “Coli-Inspector.” (3)For each cell the fluorescence profile along the cell axis (see Figures 3A,C) was subdivided in two parts left and right of the central minimum value (cf. Figures 3B,D). From either part, the center of mass was calculated from the region that exceeded the central minimum, and the distance between both was calculated.